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Nuclease BAL-31 Reaction Buffer |
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Prices are in US dollars and valid only for US orders.
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Description:
New England Biolabs supplies a reaction buffer with all of its enzymes. The buffer for Nuclease BAL-31 ensures optimal activity of the enzyme when used at a 1X concentration under the recommended reaction conditions described below.
Reagents Supplied:
2X Nuclease BAL-31 Reaction Buffer
1X Nuclease BAL-31 Reaction Buffer:
600 mM NaCl
20 mM Tris-HCl
12 mM CaCl2
12 mM MgCl2
1 mM EDTA
pH 8.0 @ 25°C
Storage Conditions
Storage Temperature:
-20°C
FAQs
- What is supplied with the Nuclease BAL-31 Reaction Buffer pack?
- What is the composition of the Nuclease BAL-31 Reaction Buffer?
- How should the NEBuffer be used?
- The buffer arrived thawed; is it and the enzyme still active?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
Companion Products
Nuclease BAL-31
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