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FAQs for Taq DNA Ligase Reaction Buffer FAQs for Buffers Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE λ DNA-BstEII Digest Taq DNA Ligase

Home > Products > Buffers > Buffers and Supplements > Taq DNA Ligase Reaction Buffer Taq DNA Ligase Reaction Buffer Catalog # Size Concentration Price Qty   B0208S 6 ml   $10.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. Reagents Supplied: 10X Taq DNA Ligase Reaction Buffer λ DNA-BstEII Digest 1X Taq DNA Ligase Reaction Buffer: 20 mM Tris-HCl 25 mM potassium acetate 10 mM Magnesium Acetate 10 mM Dithiothreitol 1 mM NAD 0.1 % Triton X-100 pH 7.6 @ 25°C Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. FAQs What is supplied with the Taq DNA Ligase Reaction Buffer pack? What is the composition of Taq DNA Ligase Reaction Buffer? How should the NEBuffer be used? The buffer arrived thawed; is it and the enzyme still active? Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. 16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation. Endonuclease Activity:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II. Reagents Sold Separately λ DNA-BstEII Digest Companion Products Taq DNA Ligase