Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Reagents Supplied: 10X E. coli DNA Ligase Reaction Buffer
1X E. coli DNA Ligase Reaction Buffer: 30 mM Tris-HCl 4 mM MgCl2 1 mM Dithiothreitol 26 µM NAD 50 µg/ml BSA
pH 8.0 @ 25°C
Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
