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FAQs for T4 RNA Ligase 1 Reaction Buffer FAQs for Buffers Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE T4 RNA Ligase 1 (ssRNA Ligase)

Home > Products > Buffers > Buffers and Supplements > T4 RNA Ligase 1 Reaction Buffer T4 RNA Ligase 1 Reaction Buffer Catalog # Size Concentration Price Qty   B0204S 6 ml   $10.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. Reagents Supplied: 10X T4 RNA Ligase 1 Reaction Buffer 1X T4 RNA Ligase 1 Reaction Buffer: 50 mM Tris-HCl 10 mM MgCl2 10 mM Dithiothreitol 1 mM ATP pH 7.8 @ 25°C Storage Conditions Storage Temperature: -20°C FAQs What is supplied with the T4 RNA Ligase 1 Reaction Buffer pack? What is the composition of T4 RNA Ligase 1 Reaction Buffer? How should the NEBuffer be used? The buffer arrived thawed; is it and the enzyme still active? Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. 16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation. Endonuclease Assay:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II. Companion Products T4 RNA Ligase 1 (ssRNA Ligase)