Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Reagents Supplied: 10X T4 DNA Ligase Reaction Buffer
1X T4 DNA Ligase Reaction Buffer: 50 mM Tris-HCl 10 mM MgCl2 10 mM Dithiothreitol 1 mM ATP
pH 7.5 @ 25°C
Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
