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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > AfeI > FAQ AfeI FAQ See the Restriction Endonucleases FAQ also. Q1: Why does AfeI's catalog number start with V instead of R? Q2: What buffers can be used for double digests? Q3: Does AfeI replave one previously sold? Q4: Are more units of AfeI required to cut supercoiled DNA than lambda DNA? Q1: Why does AfeI's catalog number start with V instead of R? A1: This enzyme is made by SibEnzyme. As a service to our customers, we occasionaly supply enzymes with unique specifications that are exclusively produced by another supplier. Q2: What buffers can be used for double digests? A2: NEBuffer 4 is very similar in composition to the supplied SEBuffer Y and gives 100% activity. NEBuffer 2 can also be used if the amount of AfeI is doubled. We do not know the activity the activity of NEB enzymes in the SEBuffer system. Q3: Does AfeI replave one previously sold? A3: Yes, this enzyme replaces Eco47III (ER0321). AfeI recognizes the same sequence AGCˆGCT, and cleaves at the same position as Eco47III. Q4: Are more units of AfeI required to cut supercoiled DNA than lambda DNA? A4: Yes, 10 units are required to cleave 1 μg pBR322.