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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > HpyCH4IV > FAQ HpyCH4IV FAQ See the Restriction Endonucleases FAQ also. Q1: Is HpyCH4IV an isoschizomer of MaeII? Q2: Are TaiI and TscI isoschizomers of HpyCH4IV? Q3: Is HpyCH4IV affected by methylation? Q1: Is HpyCH4IV an isoschizomer of MaeII? A1: Yes, it recognizes the same sequence and cleaves at the same place. Both are blocked by SssI (CG) methylase (5-methyl cytosine). Q2: Are TaiI and TscI isoschizomers of HpyCH4IV? A2: TaiI and TscI are neoschizomers of HpyCH4IV. The recognize the same site but cut at a different location. TaiI is blcoked by SssI and TscI has not been tested. Q3: Is HpyCH4IV affected by methylation? A3: Yes, it is blocked by 5-methyl cytosine which can be found in mammalian DNA. New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201