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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BaeI > FAQ BaeI FAQ See the Restriction Endonucleases FAQ also. Q1: Does BaeI have any special requirements? Q2: Does BaeI cleave DNA twice? Q1: Does BaeI have any special requirements? A1: Requires S-adenosylmethionine for optimal activity (supplied with enzyme). Q2: Does BaeI cleave DNA twice? A2: BaeI cleaves DNA substrates twice to excise its recognition site generating a 28 base-pair fragment with 5-base 3´ overhangs.