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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > Acc65I > FAQ Acc65I FAQ See the Restriction Endonucleases FAQ also. Q1: Is Acc65I an isoschizomer of KpnI? Q2: What factors inhibit Acc65I activity? Q1: Is Acc65I an isoschizomer of KpnI? A1: Yes, but it leaves a different cohesive end. KpnI produces a 4-base 3´ extension, whereas Acc65I leaves a 4-base 5´ extension. Q2: What factors inhibit Acc65I activity? A2: Overlapping dcm methylation. Dcm CC(A/T)GG blocks Acc65I when the sequence is GGTACC(A/T)GG or CC(A/T)GGTACC.