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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BseRI > FAQ BseRI FAQ See the Restriction Endonucleases FAQ also. Q1: Is extended digestion of BseRI recommended? Q2: Can over digestion be detrimental? Q3: Does BseRI have a single site in a common vector? Q4: What is the activity of BseRI at 25°C? Q1: Is extended digestion of BseRI recommended? A1: Extended digestion is not beneficial because the enzyme is not stable in reaction. Q2: Can over digestion be detrimental? A2: BseRI has an exonuclease or nonspecific endonuclease activity that appears to be a property of the enzyme. Do not use greater than 10 units per ug of DNA. Q3: Does BseRI have a single site in a common vector? A3: Yes, there is a single BseRI site in M13mp18. Q4: What is the activity of BseRI at 25°C? A4: BseRI is 25-50% active at 25°C.