A1: Bsp1497I was replaced by BsrGI, (NEB #R0575). BsrGi is an isoschizomer of Bsp1407I and cleaves at the same position in the recognition sequence Tˆ GTACA.
Q2: How can the activity be increased?
A2: Incubation of BsrGi at 60°C results in a 2-fold increase in activity.