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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BsmFI > FAQ BsmFI FAQ See the Restriction Endonucleases FAQ also. Q1: Are there single cleavage sites in any common vectors for BsmFI? Q2: Is there variability in the cleavage site for BsmFI? Q3: Is activity loss seen in 12 months or less? Q4: What is the activity of BsmFI at 37°C? Q1: Are there single cleavage sites in any common vectors for BsmFI? A1: Yes. The polylinkers found in pACYC177 cloning vectors contain a single BsmFI site. Q2: Is there variability in the cleavage site for BsmFI? A2: Yes. BsmFI recognizes GGGAC and cleaves 10/14 bases downstream, but sometimes cleaves 9/13. Q3: Is activity loss seen in 12 months or less? A3: Yes, in 12 months. Q4: What is the activity of BsmFI at 37°C? A4: BsmFI is anisoschizomer of FinI. Incubation at 37°C results in 50% activity.