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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > AscI > FAQ AscI FAQ See the Restriction Endonucleases FAQ also. Q1: What factors inhibit AscI? Q1: What factors inhibit AscI? A1: AscI is strongly inhibited by certain salts, including NaCl or Ammonium acetate. For alcohol precipitation before digestion, we recommend using Sodium Acetate (or Potassium Acetate) as a salt in the precipitation. (Note that NEBuffer 4 conatins potassium acetate.)