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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > AciI > FAQ AciI FAQ See the Restriction Endonucleases FAQ also. Q1: Why can only 50% of AciI cut and ligated fragments be recleaved by AciI? Q1: Why can only 50% of AciI cut and ligated fragments be recleaved by AciI? A1: AciI has a non-palindromic recognition site. As a result, when DNA is cut with AciI and then ligated, only 50% of these ligated sites regenerate an AciI site.