A1: BcgI requires SAM for activity. SAM is only stable for six months when stored at -20°C. Fresh SAM can be purchased from us; ask for product number B9003S.
Q2: Does BcgI require SAM for activity?
A2: Yes, 20 µM SAM is required for optimal activity.
Q3: Do BcgI generated ends ligate?
A3: BcgI excises a 32 bp fragment with 2-base 3´ overhangs. These overhangs are not specific, so whether the ends can be ligated depends entirely on the specific DNA sequence being cut.
Q4: What is the molecular weight of BcgI?
A4: The MW of BcgI is 180 kD estimated from PAGE. Kong et al. (1994) Journal of Biological Chemistry 269, 683-690.
