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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BspEI > FAQ BspEI FAQ See the Restriction Endonucleases FAQ also. Q1: Why isn't BspEi cutting? Q2: Does BspEI produce commonly used compatible ends? Q3: Does BspEI replace one previously sold? Q1: Why isn't BspEi cutting? A1: 1. BspEI requires fresh DDT in the reaction buffer. 2. BspEI has trouble cleaving PCR products, but it is improved by addition of 1X assay buffer or 100mM NaCl. 3. BspEI is blocked by dam methylation. 4. BspEI is slowed by CpG methylation. Q2: Does BspEI produce commonly used compatible ends? A2: BspEI cleaves to leave a 5´ GGCC which can be ligated to fragments generated by AgeI, BsaWI, BsrFi, AvaI, XmaI, NgoMI digestion. Q3: Does BspEI replace one previously sold? A3: Yes, BspEI replaces BspMII.