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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BbsI > FAQ BbsI FAQ See the Restriction Endonucleases FAQ also. Q1: Are storage conditions for BbsI other than -20°C recommended? Q2: Is activity loss of BbsI seen in 12 months or less? Q3: Does salt inhibit BbsI cleavage? Q4: What is the activity of BbsI at 25°C? Q1: Are storage conditions for BbsI other than -20°C recommended? A1: Yes, -70°C for periods longer than 30 days, otherwise store at -20°C. Q2: Is activity loss of BbsI seen in 12 months or less? A2: Yes, loss can be seen after 12 moths storage at -20°C. Q3: Does salt inhibit BbsI cleavage? A3: Mini-prep DNA containing residual salt is resistant to BbsI cleavage. A 70% ethanol wash or dialysis should be performed to remove the salt. Q4: What is the activity of BbsI at 25°C? A4: BbsI is 0-25% active at 25°C.