A1: BsmAI itself is very stable but needs fresh DTT. The DTT often needs spiking if the buffer is older than 6 months.
Q2: Does BsmAI replace an enzyme previously sold?
A2: Yes, Alw26I.
Q3: Is BsmAI blocked by CpG methylation?
A3: Yes, at sites with overlapping CG.
Q4: What is the activity level of BsmAI at 37°C?
A4: Incubation at 37°C results in 30% activity and at 65°C results in 10% activity.
Q5: Does BsmAI exhibit reduced activity on supercoiled DNA?
A5: Yes, pUC19 has been determined to need at least a 2X overdigestion.
Q6: Is overnight digestion with BsmAI recommended?
A6: 16 hour incubations with BsmAI are not recommended because the enzyme loses activity during the reaction. Like most type IIs enzymes, BsmAI does not turn over very well, therefore it is preferable to add more units for a shorter period of time.
