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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BsrI > FAQ BsrI FAQ See the Restriction Endonucleases FAQ also. Q1: Are there any known sequence errors at the recognition site in a database? Q2: Does BsrI have trouble cleaving PCR products? Q3: What is the activity of BsrI at 37°C? Q1: Are there any known sequence errors at the recognition site in a database? A1: Yes, BsrI cleaves Bluescript 13 times not once as previously reported in Stratagene catalog. Q2: Does BsrI have trouble cleaving PCR products? A2: Yes. PCR products should be phenol extracted or column purifed, and EtOH precipitated, before cleaving with BsrI. Q3: What is the activity of BsrI at 37°C? A3: Incubation at 37°C results in 20% activity.