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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BspHI > FAQ BspHI FAQ See the Restriction Endonucleases FAQ also. Q1: Why isn't BspHI cutting? Q2: What is the molecular weight of BspHI? Q3: What is the activity of BspHI at 25°C? Q1: Why isn't BspHI cutting? A1: BspHI is blocked by overlapping dam methylation. Excess salt can also be a problem. If this may be the case, we recommend to drop dialyze minipreps before cleavage. Q2: What is the molecular weight of BspHI? A2: The MW of BspHI is 43,719 daltons from sequence data. Q3: What is the activity of BspHI at 25°C? A3: BspHI is 75-100% active at 25°C.