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FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > EagI > FAQ EagI FAQ See the Restriction Endonucleases FAQ also. Q1: Does EagI produce commonly used compatible ends? Q2: IsEagI pH sensitive? Q3: Are more units of EagI required to cut supercoiled DNA than lambda DNA? Q1: Does EagI produce commonly used compatible ends? A1: EagI-cut DNA can be ligated to DNA cut with NotI or EaeI. Q2: IsEagI pH sensitive? A2: EagI is less active when used at lower then recommended pH. Q3: Are more units of EagI required to cut supercoiled DNA than lambda DNA? A3: Yes, 10 units are required to cleave 1 ug of litmus or pBR322 DNA.