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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > SphI > FAQ SphI FAQ See the Restriction Endonucleases FAQ also. Q1: Are more units of SphI required to cut supercoiled DNA than lambda DNA? Q2: What is the activity of SphI at 25°C? Q3: Does SphI produce commonly used compatible ends? Q4: What is the molecular weight of SphI? Q1: Are more units of SphI required to cut supercoiled DNA than lambda DNA? A1: Yes, 4 units are required to cleave 1 μg of litmus DNA, 2 units are required for pBR322 and 3 units for pUC19. Q2: What is the activity of SphI at 25°C? A2: SphI is 50-75% active at 25°C. Q3: Does SphI produce commonly used compatible ends? A3: Celaves to leave a 3´ CATG extension which can be efficiently ligated to DNA fragments generated by NlaIII. Q4: What is the molecular weight of SphI? A4: The MW of SphI is 29 kDa as estimated by PAGE.