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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Restriction Endonucleases > Restriction Endonucleases > BbvI > FAQ BbvI FAQ See the Restriction Endonucleases FAQ also. Q1: Are there any special considerations when working with BbvI? Q2: Does BbvI cleave ssDNA? Q1: Are there any special considerations when working with BbvI? A1: Like many type IIS (shifted cut site) restriction enzymes, BbvI seems to remain bound to its substrate after cleavage. Extended digests are not recommended because 1 unit is required to cleave 1 μg of DNA in 16 hours. Phenol extraction is the suggested method of DNA purification due to the binding properties of the enzyme. Q2: Does BbvI cleave ssDNA? A2: No.