A2: SalI activity decreases if the reaction buffer pH is not between 7.8 and 8.0.
Q3: Is SalI affected by star activity?
A3: Yes, high glycerol, high enzyme concentration, or long reaction times can cause star activity of SalI.
Q4: Are extended digestions with SalI recommended?
A4: Greater than 4 hour digestions are not recommended.
Q5: Is SalI inhibited by nucleotides?
A5: Yes.
Q6: Does SalI exhibit reduced activity on supercoiled DNA?
A6: Supercoiled plasmids may require up to 10-fold more SalI for complete digestion than on linear DNA.
Q7: What is the activity of SalI at 25°C?
A7: It is 50-75% active.
Q8: Does SalI have trouble cleaving PCR products?
A8: SalI has trouble cleaving PCR products.
Q9: What is the molecular weight of SalI?
A9: 30 kDa.
Q10: Are more units of SalI required to cut supercoiled DNA than lambda DNA?
A10: Supercoiled limus, pBR322 and pUC plasmids require up to 10 times more enzyme to achieve complete digestion.
Q11: Are SalI buffer and BamHI buffer identical?
A11: Yes.
Q12: How many bases does SalI require for cleavage close to the ends?
A12: When cleaving close to the ends of DNA fragments, cleavage should be done at 37°C for one hour using 10 units/μg of DNA with a minimum of 3 bases on each side of the recognition sequence.
