To access your account, log in or register.	shopping cart | log in

Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Protein Tools > Protein Kinases/Substrates/Inhibitors > cAMP-dependent Protein Kinase (PKA), catalytic subunit > FAQ cAMP-dependent Protein Kinase (PKA), catalytic subunit FAQ See the Protein Tools FAQ also. Q1: How much cAMP-dependent Protein Kinase (NEB# P6000) should be used? Q2: What is the consensus sequence for PKA (P6000)? Q1: How much cAMP-dependent Protein Kinase (NEB# P6000) should be used? A1: The amount of enzyme required varies when different substrates are used. Start with 1-2 µl for 1µg of protein for one hour in a 10-25 µl reaction. If there is still unphosphorylated material, let the reaction go overnight. Q2: What is the consensus sequence for PKA (P6000)? A2: The consensus sequence is RRXS/TY, where Y tends to be a hydrophobic residue. A phenylalanine in the nearby sequences tends to be a negative determinant for phosphorylation by PKA