A1: Four vials, one containing dATP, dCTP, dGTP, and dTTP.
Q2: How much is in each vial of the Deoxynucleotide Solution Set?
A2: Each vial contains 25 μmol in a volume of 0.25 mls. A small pack contains one vial of each.
Q3: What is the concentration of dNTPs in the Deoxynucleotide Solution Set?
A3: The concentration is 100mM.
Q4: Which polymerases can the Deoxynucleotide Solution Set be used with?
A4: Any DNA Polymerase from NEB.
Q5: How does this product differ from the Deoxynucleotide Solutions Mix (NEB# N0447)?
A5: The nucleotides are mixed at a concentration of 10 mM of each dNTP in the Deoxynucleotide Solutions Mix. If equal volumes of nucleotides are mixed from the Deoxynucleotide Solutions Set the concentration would be 25 mM each dNTP.
Q6: How stable are the nucleotides in the Deoxynucleotide Solution Set?
A6: When stored at -20°C, they are stable for years. If they are freeze thawed frequently they should be stable for a year. If the solutions are stored at 4°C bacteria can grow in them. Bacterial growth is accelerated if the solution is diluted in a buffer containing magnesium. Discard solutions stored at 4°C after one week.
