A2: Yes. UDG will work well in T4 DNA ligase buffer.
Q3: What is the molecular weight of UDG?
A3: The molecular weight of UDG is 25.7 kDa.
Q4: Is UDG a tagged protein?
A4: No. UDG is not a tagged protein.
Q5: I see that UDG works optimally at 37°C. Do you have another glycosylase that works at higher temperatures?
A5: Yes. Afu UDG (M0279) can release uracil from a uracil-containing DNA at 65°C.
Q6: Does the Uracil Glycosylase inhibitor (UGI) inhibit UDG?
A6: Yes. Under standard conditions UGI will inhibit the activity of UDG.
Q7: Does UDG release uracil from ss and dsDNA?
A7: Yes. UDG can remove uracils from both ss and dsDNA.
Q8: Does UDG cut RNA?
A8: No, UDG should not act on an RNA substrate. UDG evolved to repair dU residues in DNA that can be created by deamination of dC (hence avoiding the mutation of a C:G base pair to T:A that would occur when the U-strand is replicated).
Q9: Can UDG be used to remove dU from a short 21mer oligo? Do the dU residues need to be spaced in any special way to avoid problems with cleavage?
A9: UDG should work fine on a 21mer. Spacing is not important.
Q10: Are there any specific recommendations for the use of UDG on single stranded DNA? The materials on the web and data card seem to describe conditions for use with dsDNA.
A10: UDG acts equally well in ssDNA and dsDNA so the usage recommendations are the same for both types of substrates.
Q11: What is the difference between UDG and UNG?
A11: They are the same protein. UNG is the actual E. coli gene name.
