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Home > Products > RNA Reagents > RNases > Exonuclease T > FAQ Exonuclease T FAQ See the RNA Reagents FAQ also. Q1: Will Exonuclease T degrade single stranded RNA? Q2: Can Exonuclease T be heat inactivated? Q3: What is the activity of Exonuclease T in the NEBuffers? Q4: What is the molecular weight of Exonuclease T? Q5: Does Exonuclease T work as well as DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) for 3' extension removal? Q6: How is Exonuclease T different from Exonuclease I (NEB# M0293)? Q1: Will Exonuclease T degrade single stranded RNA? A1: Yes. Exonuclease T is also known as RNase T. Q2: Can Exonuclease T be heat inactivated? A2: Yes. Heat at 65°C for 20 minutes. Q3: What is the activity of Exonuclease T in the NEBuffers? A3: NEBuffer 1: 100% NEBuffer 2: 75% NEBuffer 3: 25% NEBuffer 4: 100% Q4: What is the molecular weight of Exonuclease T? A4: 24 kD Q5: Does Exonuclease T work as well as DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) for 3' extension removal? A5: DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) or T4 DNA Polymerase (NEB# M0203) are preferred. They do, however, require the addition of nucleotides for 3' extension removal. Q6: How is Exonuclease T different from Exonuclease I (NEB# M0293)? A6: Exonuclease I will not remove short 3' extensions.