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Home > Products > DNA Modifying Enzymes and Cloning > Nucleases > Nuclease BAL-31 > FAQ Nuclease BAL-31 FAQ See the DNA Modifying Enzymes and Cloning FAQ also. Q1: Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment? Q2: Why does all of the DNA get degraded when I use Nuclease BAL-31? Q3: Can Nuclease BAL-31 be heat inactivated? Q4: Is Nuclease BAL-31 active in other NEBuffers? Q5: Can Nuclease BAL-31 treated DNA be cloned? Q6: What is a good control for the BAL-31 nuclease? Q7: Will Nuclease BAL-31 degrade RNA? Q1: Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment? A1: No. The reaction is difficult to control. A minimum of 50 base pairs will be removed. Q2: Why does all of the DNA get degraded when I use Nuclease BAL-31? A2: More DNA needs to be added to the reaction or the enzyme needs to be diluted with reaction buffer. Q3: Can Nuclease BAL-31 be heat inactivated? A3: Yes, in the presence of EDTA or EGTA. Heat inactivate at 65°C for 20 min. Q4: Is Nuclease BAL-31 active in other NEBuffers? A4: No. BAL-31 requires very high salt and calcium. Q5: Can Nuclease BAL-31 treated DNA be cloned? A5: After purification followed by Klenow treatment the DNA can be cloned using blunt ends. Q6: What is a good control for the BAL-31 nuclease? A6: A time course using a DNA marker works well as a control. Q7: Will Nuclease BAL-31 degrade RNA? A7: Yes.