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DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit > FAQ |  | DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit FAQ
See the Polymerases and Amplification FAQ also.
Q1: What applications are the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits recommended for?
Q2: What are the advantages to using the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
Q3: What reverse transcriptase is used in the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
Q4: What instruments can the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits be used with?
Q5: Are Finnzymes' DNA Polymerases licensed for PCR?
Q1: What applications are the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits recommended for?
A1: They are recommended for the detection and quantification of RNA with great sensitivity and specificity.
Q2: What are the advantages to using the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
A2: Broad dynamic range - up to 1 ug of RNA
Versatility - reliable results with mRNA, total RNA, viral RNA and in vitro transcribed RNA
Flexibility - two-step system, all priming options
Ease of use - optimized reaction mixes and short protocols
Specific and Sensitive
Q3: What reverse transcriptase is used in the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
A3: M-MuLV RNase H+, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases. The RNase H activity in the RT enzyme facilitates annealing of PCR primers to the cDNA by degrading the RNA template from the RNA-cDNA hybrid before the PCR step.
Q4: What instruments can the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits be used with?
A4: DyNAmo SYBR Green 2-Step qRT-PCR Kit (F-430) is compatible with all block-based real-time qPCR instruments and DyNAmo Capillary SYBR Green 2-Step qRT-PCR Kit (F-440) is engineered for capillary based instruments.
Q5: Are Finnzymes' DNA Polymerases licensed for PCR?
A5: Yes.
Phusion, DyNAzyme and DyNAmo are trademarks of Finnzymes Oy. SYBR is a registered trademark of Molecular Probes, Inc.
PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD, Roche Molecular Systems, Inc. and The Perkin-Elmer Corporation. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Perkin-Elmer or as purchased, i.e. an authorized thermal cycler. Notice to purchaser: Limited license. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by New England Biolabs, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. Use of DyNAmo Probe qPCR Kits may also require licenses from other third parties such as, but not limited to, the owners of hybridization probes and molecular beacons. Purchase of DyNAmo Probe qPCR Kits does not provide such licenses and further information on purchasing licenses to practice this technology must be obtained from the appropriate intellectual property owners of such technology.
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