A2: Diluent Buffer B: 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 500 µg/ml BSA, 50 % glycerol, pH 7.4 at 25 °C.
Q3: How should the Diluent Buffer be used?
A3: Dilute enzymes just prior to use keeping the final concentration of diluted enzymes at least 1,000 units/ml. Diluent preference for each restriction endonuclease is listed with its catalog entry and on its data card.
Q4: How long can the diluted enzyme be stored?
A4: The stability of each enzyme diluted below selling concentration has not been tested. Many enzymes will remain active for long periods of time when stored diluted at -20°C. Use a control substrate to test dilutions that have been stored.
