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FAQ |  | Protein Tools FAQ
To see FAQs about a specific Protein Tools, see links from the specific product's page.
Q1: What is Ph.D.™ Phage Display?
Q2: What peptide libraries are available for use with Ph.D.™ Phage Display?
Q3: What products has NEB developed from ongoing basic research in molecular parasitology?
Q4: What are Glycosidases and their uses?
Q1: What is Ph.D.™ Phage Display?
A1: It is a selection technique in which a library of variants of a peptide or protein is expressed on the outside of a phage virion, while the genetic material encoding each variant resides on the inside. This creates a physical linkage between each variant protein sequence and the DNA encoding it, which allows rapid partitioning based on binding affinity to a given target molecule (antibodies, enzymes, cell-surface receptors, etc.) by an in vitro selection process called panning.
(1) Sidhu, S.S. et al. (2003) Chembiochem. 4, 14-25.
(2) Azzazy, H.M. and Highsmith, W.E. (2002) Clin. Biochem. 35, 425-445.
(3) Rodi, D.J. et al. (200) Curr. Opin. Chem. Biol. 6, 92-96.
Q2: What peptide libraries are available for use with Ph.D.™ Phage Display?
A2: NEB offers 3 pre-made random peptide libraries, as well as the cloning vector M13KE (NEB# E8101S) for construction of custom libraries. The pre-made libraries consist of linear heptapeptide (Ph.D.™ -7 Library NEB# E8102L) and dodecapeptide (Ph.D.™-12 Library NEB# E8111L) libraries, as well as a disulfide-constrained heptapeptide (Ph.D.™-C7C Library NEB# E8121L) library.
Q3: What products has NEB developed from ongoing basic research in molecular parasitology?
A3: For more than twenty years, NEB has supported basic research in molecular parasitology. It is hoped that this research may contribute towards the development of technologies that can control filarial parasites and therefore improve the quality of life in areas where filarial infections are endemic. The parasitology group at NEB has been working, in collaboration with a number of scientists from various universities and institutions, to discover new potential targets for anti-parasite chemotherapy. We have made available several molecular targets for further investigation, including peptidyl-prolyl cis-trans isomerases (PPIases), chitinases and proteases.
Q4: What are Glycosidases and their uses?
A4: Glycosidases are used to get information about the carbohydrate groups attached to glycoproteins and glycopeptides. They come in two varieties, endoglycosidases that cleave entire carbohydrate groups from proteins and exoglycosidases that remove monosaccharides from the nonreduced ends of the carbohydrate. A reduced end is one generated by an endoglycosidase. Researchers frequently use an endoglycosidase followed by one or more exoglycosidases and then analyze the products using SDS-PAGE or various types of liquid chromatography.
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