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FAQ |  | Gene Expression and Protein Purification FAQ
To see FAQs about a specific Gene Expression and Protein Purification, see links from the specific product's page.
Q1: What systems does NEB offer for protein expression and purification?
Q1: What systems does NEB offer for protein expression and purification?
A1: The pMAL™ Protein Fusion and Purification System (NEB# E8000S) is available for producing a protein expressed from a cloned gene or open reading frame. The gene of interest is fused to the malE gene, which encodes maltose-binding protein (MBP), and expressed at levels constituting up to 40% of the total cell protein using the tac promoter and translation initiation signals of MBP. A one-step affinity purification for MBP is used to isolate the fusion protein.
The IMPACT™ System (NEB# E6901S) utilizes engineered protein splicing elements (inteins) to purify recombinant proteins by a single affinity column. This system distinguishes itself from other protein fusion systems by its ability to separate a recombinant protein from the affinity tag without the use of a protease. In addition, native recombinant proteins possessing a C-terminal thioester can be isolated for applications such as protein semisynthesis and site specific labeling.
The K.lactis Protein Expression Kit (NEB# E1000S) provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or be secreted using the supplied integrative expression vector pKLAC1.
The RheoSwitch® Mammalian Inducible Expression System (NEB# E3000S) represents the next generation of inducible gene expression systems for mammalian cells. Analogous to the operation of a rheostat, the RheoSwitch technology allows induction and adjustable control of gene expression. Regulation of gene expression is achieved through the highly specific interaction of a synthetic inducer, RheoSwitch Ligand RSL1, and a chimeric bipartite nuclear receptor. This receptor is activated in the presence of RSL1 ligand, and the level of gene expression can be regulated by adjusting the concentration of RSL1 ligand in the tissue culture media.
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