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FAQ |  | Protein Tools FAQ
To see FAQs about a specific Protein Tools, see links from the specific product's page.
Q1: What are the advantages of magnetic affinity matrices?
Q2: What are Glycosidases and their uses?
Q1: What are the advantages of magnetic affinity matrices?
A1: Magnetic particles are ideally suited for applications involving high-throughput proteomic screening, small-scale protein isolation, immunomagnetic isolations or cell separation experiments. With magnetic beads, affinitly purification of tagged proteins, antigens, antibodies and nucleic acids can be done conveniently and quickly, with minimal time necessary for separation of the solid-phase from solution. In addition, immobilized substrates remain biologically active and can be eluted in samll volumes or serve as ligands in subsequent pull-down or target interaction experiments involving DNA or proteins.
There are several additional advantages to this method. No centrifugation is required. The matrix can be regenerated without loss of binding capacity. There is also minimal sample loss during pipetting because magnetic beads concentrate at the side of the tube instead of the bottom.
Q2: What are Glycosidases and their uses?
A2: Glycosidases are used to get information about the carbohydrate groups attached to glycoproteins and glycopeptides. They come in two varieties, endoglycosidases that cleave entire carbohydrate groups from proteins and exoglycosidases that remove monosaccharides from the nonreduced ends of the carbohydrate. A reduced end is one generated by an endoglycosidase. Researchers frequently use an endoglycosidase followed by one or more exoglycosidases and then analyze the products using SDS-PAGE or various types of liquid chromatography.
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