 |
|
|
Enzyme |
|
Sequence |
|
Cut Site |
|
Overhang |
|
Properties (NEB Enzymes Only) |
|
|
|
|
|
Nb.BbvCI |
|
CCTCAGC |
|
| C |
|
C |
|
T |
|
C |
|
A |
|
G |
|
C |
|
|
| G |
|
G |
|
A |
|
G |
|
T |
/ |
C |
|
G |
|
|
|
|
|
|
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|
|
|
|
|
|
|
|
|
nick |
|
 |
|
 |
|
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|
 |
|
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|
|
|
 |
|
 |
|
|
|
|
|
|
|
|
|
|
BbvCI |
|
CCTCAGC |
|
| C |
|
C |
/ |
T |
|
C |
|
A |
|
G |
|
C |
| G |
|
G |
|
A |
|
G |
|
T |
/ |
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - TCA |
|
|
|
|
|
 |
|
 |
|
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|
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|
|
|
|
|
|
|
|
Nt.BbvCI |
|
CCTCAGC |
|
| C |
|
C |
/ |
T |
|
C |
|
A |
|
G |
|
C |
| G |
|
G |
|
A |
|
G |
|
T |
|
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
nick |
|
|
|
|
|
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|
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|
|
|
AscI |
|
GGCGCGCC |
|
| G |
|
G |
/ |
C |
|
G |
|
C |
|
G |
|
C |
|
C |
| C |
|
C |
|
G |
|
C |
|
G |
|
C |
/ |
G |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - CGCG |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
AscI RE-Mix™ |
|
GGCGCGCC |
|
| G |
|
G |
/ |
C |
|
G |
|
C |
|
G |
|
C |
|
C |
| C |
|
C |
|
G |
|
C |
|
G |
|
C |
/ |
G |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - CGCG |
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
AsiSI |
|
GCGATCGC |
|
| G |
|
C |
|
G |
|
A |
|
T |
/ |
C |
|
G |
|
C |
| C |
|
G |
|
C |
/ |
T |
|
A |
|
G |
|
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
AT - 3´ |
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
FseI |
|
GGCCGGCC |
|
| G |
|
G |
|
C |
|
C |
|
G |
|
G |
/ |
C |
|
C |
| C |
|
C |
/ |
G |
|
G |
|
C |
|
C |
|
G |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CCGG - 3´ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
NotI |
|
GCGGCCGC |
|
| G |
|
C |
/ |
G |
|
G |
|
C |
|
C |
|
G |
|
C |
| C |
|
G |
|
C |
|
C |
|
G |
|
G |
/ |
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - GGCC |
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
NotI-HF™ RE-Mix™ |
|
GCGGCCGC |
|
| G |
|
C |
/ |
G |
|
G |
|
C |
|
C |
|
G |
|
C |
| C |
|
G |
|
C |
|
C |
|
G |
|
G |
/ |
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - GGCC |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
NotI-HF™ |
|
GCGGCCGC |
|
| G |
|
C |
/ |
G |
|
G |
|
C |
|
C |
|
G |
|
C |
| C |
|
G |
|
C |
|
C |
|
G |
|
G |
/ |
C |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
5´ - GGCC |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PacI |
|
TTAATTAA |
|
| T |
|
T |
|
A |
|
A |
|
T |
/ |
T |
|
A |
|
A |
| A |
|
A |
|
T |
/ |
T |
|
A |
|
A |
|
T |
|
T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
AT - 3´ |
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PacI RE-Mix™ |
|
TTAATTAA |
|
| T |
|
T |
|
A |
|
A |
|
T |
/ |
T |
|
A |
|
A |
| A |
|
A |
|
T |
/ |
T |
|
A |
|
A |
|
T |
|
T |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
AT - 3´ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PmeI |
|
GTTTAAAC |
|
| G |
|
T |
|
T |
|
T |
/ |
A |
|
A |
|
A |
|
C |
| C |
|
A |
|
A |
|
A |
/ |
T |
|
T |
|
T |
|
G |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
blunt |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SbfI |
|
CCTGCAGG |
|
| C |
|
C |
|
T |
|
G |
|
C |
|
A |
/ |
G |
|
G |
| G |
|
G |
/ |
A |
|
C |
|
G |
|
T |
|
C |
|
C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
TGCA - 3´ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
SbfI-HF™ |
|
CCTGCAGG |
|
| C |
|
C |
|
T |
|
G |
|
C |
|
A |
/ |
G |
|
G |
| G |
|
G |
/ |
A |
|
C |
|
G |
|
T |
|
C |
|
C |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
TGCA - 3´ |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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|
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|
|
