 |
 |
 |
Enzyme |
 |
Sequence |
 |
Cut Site |
 |
Overhang |
 |
Properties (NEB Enzymes Only) |
 |
 |
 |
 |
 |
Nb.BbvCI |
 |
CCTCAGC |
 |
| C |
 |
C |
 |
T |
 |
C |
 |
A |
 |
G |
 |
C |
 |
|
| G |
 |
G |
 |
A |
 |
G |
 |
T |
/ |
C |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
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|
 |
nick |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
BbvCI |
 |
CCTCAGC |
 |
| C |
 |
C |
/ |
T |
 |
C |
 |
A |
 |
G |
 |
C |
| G |
 |
G |
 |
A |
 |
G |
 |
T |
/ |
C |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
5´ - TCA |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
Nt.BbvCI |
 |
CCTCAGC |
 |
| C |
 |
C |
/ |
T |
 |
C |
 |
A |
 |
G |
 |
C |
| G |
 |
G |
 |
A |
 |
G |
 |
T |
 |
C |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
nick |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
AscI |
 |
GGCGCGCC |
 |
| G |
 |
G |
/ |
C |
 |
G |
 |
C |
 |
G |
 |
C |
 |
C |
| C |
 |
C |
 |
G |
 |
C |
 |
G |
 |
C |
/ |
G |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
5´ - CGCG |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
AsiSI |
 |
GCGATCGC |
 |
| G |
 |
C |
 |
G |
 |
A |
 |
T |
/ |
C |
 |
G |
 |
C |
| C |
 |
G |
 |
C |
/ |
T |
 |
A |
 |
G |
 |
C |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
AT - 3´ |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
FseI |
 |
GGCCGGCC |
 |
| G |
 |
G |
 |
C |
 |
C |
 |
G |
 |
G |
/ |
C |
 |
C |
| C |
 |
C |
/ |
G |
 |
G |
 |
C |
 |
C |
 |
G |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
CCGG - 3´ |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
NotI |
 |
GCGGCCGC |
 |
| G |
 |
C |
/ |
G |
 |
G |
 |
C |
 |
C |
 |
G |
 |
C |
| C |
 |
G |
 |
C |
 |
C |
 |
G |
 |
G |
/ |
C |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
5´ - GGCC |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
PacI |
 |
TTAATTAA |
 |
| T |
 |
T |
 |
A |
 |
A |
 |
T |
/ |
T |
 |
A |
 |
A |
| A |
 |
A |
 |
T |
/ |
T |
 |
A |
 |
A |
 |
T |
 |
T |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
AT - 3´ |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
PmeI |
 |
GTTTAAAC |
 |
| G |
 |
T |
 |
T |
 |
T |
/ |
A |
 |
A |
 |
A |
 |
C |
| C |
 |
A |
 |
A |
 |
A |
/ |
T |
 |
T |
 |
T |
 |
G |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
blunt |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
SbfI |
 |
CCTGCAGG |
 |
| C |
 |
C |
 |
T |
 |
G |
 |
C |
 |
A |
/ |
G |
 |
G |
| G |
 |
G |
/ |
A |
 |
C |
 |
G |
 |
T |
 |
C |
 |
C |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
TGCA - 3´ |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
SgrAI |
 |
CRCCGGYG |
 |
| C |
 |
R |
/ |
C |
 |
C |
 |
G |
 |
G |
 |
Y |
 |
G |
| G |
 |
Y |
 |
G |
 |
G |
 |
C |
 |
C |
/ |
R |
 |
C |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
5´ - CCGG |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
SwaI |
 |
ATTTAAAT |
 |
| A |
 |
T |
 |
T |
 |
T |
/ |
A |
 |
A |
 |
A |
 |
T |
| T |
 |
A |
 |
A |
 |
A |
/ |
T |
 |
T |
 |
T |
 |
A |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
|
 |
blunt |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
 |
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 |
 |
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 |
 |
 |
 |
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