Enzyme Finder -- Nicking, NEB

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	Enzyme Finder -- Nicking

Enzyme

Sequence

Cut Site

Overhang

Properties (NEB Enzymes Only)

CCD

	/	C		C		D
		G		G		H

nick

Cloned

Recombinant

NEBuffer 4

Temperature 37

Can be heat inactivated

GAATGC

G

A

A

T

G

C

C

T

T

A

C

/

G

nick

Cloned

Recombinant

NEBuffer 3

Temperature 65

Can be heat inactivated

CCTCAGC

C

C

T

C

A

G

C

G

G

A

G

T

/

C

G

nick

Cloned

Recombinant

NEBuffer 2

Temperature 37

Can be heat inactivated

GCAATG

G

C

A

A

T

G

N

C

G

T

T

A

C

/

N

nick

Cloned

Recombinant

NEBuffer 2

Temperature 65

Can be heat inactivated

GCAGTG

G

C

A

G

T

G

N

C

G

T

C

A

C

/

N

nick

Cloned

Recombinant

NEBuffer 4

BSA

Temperature 37

Can be heat inactivated

CCTCAGC

C

C

/

T

C

A

G

C

G

G

A

G

T

C

G

nick

Cloned

Recombinant

NEBuffer 4

Temperature 37

Can be heat inactivated

GGATC

G

G

A

T

C

N

N

N

N

/

C

C

T

A

G

N

N

N

N

nick

Cloned

Recombinant

NEBuffer 2

Temperature 37

Can be heat inactivated

dam sensitive

GAGTC

G

A

G

T

C

N

N

N

N

/

C

T

C

A

G

N

N

N

N

nick

Cloned

Recombinant

NEBuffer 3

Temperature 55

Can be heat inactivated