Double Digest Finder

Cleaving a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Use this tool to select reaction conditions amenable to any two NEB restriction enzymes. Click here for more information on double digests and a chart of double digests for some common enzyme combinations. To perform a double digest calculation, please select both 1st and 2nd enzymes. Select 1st enzyme:      AatIIAcc65IAccIAciIAclIAcuIAfeIAflIIAflIIIAgeIAgeI-HF™AhdIAleIAluIAlwIAlwNIApaIApaLIApeKIApoIAscIAseIAsiSIAvaIAvaIIAvrIIBaeGIBaeIBamHIBamHI-HF™BanIBanIIBbsIBbvCIBbvIBccIBceAIBcgIBciVIBclIBcoDIBfaIBfuAIBfuCIBglIBglIIBlpIBmgBIBmrIBmtIBmtI-HF™BpmIBpu10IBpuEIBsaAIBsaBIBsaHIBsaIBsaI-HF™BsaJIBsaWIBsaXIBseRIBseYIBsgIBsiEIBsiHKAIBsiWIBslIBsmAIBsmBIBsmFIBsmIBsoBIBsp1286IBspCNIBspDIBspEIBspHIBspMIBspQIBsrBIBsrDIBsrFIBsrGIBsrIBssHIIBssKIBssSIBstAPIBstBIBstEIIBstEII-HF™BstNIBstUIBstXIBstYIBstZ17IBsu36IBtgIBtgZIBtsCIBtsIBtsIMutICac8IClaICspCICviAIICviKI-1CviQIDdeIDpnIDpnIIDraIDraIIIDraIII-HF™DrdIEaeIEagIEagI-HF™EarIEciIEco53kIEcoNIEcoO109IEcoP15IEcoRIEcoRI-HF™EcoRVEcoRV-HF™FatIFauIFnu4HIFokIFseIFspEIFspIHaeIIHaeIIIHgaIHhaIHincIIHindIIIHindIII-HF™HinfIHinP1IHpaIHpaIIHphIHpy166IIHpy188IHpy188IIIHpy99IHpyAVHpyCH4IIIHpyCH4IVHpyCH4VKasIKpnIKpnI-HF™LpnPIMboIMboIIMfeIMfeI-HF™MluCIMluIMlyIMmeIMnlIMscIMseIMslIMspA1IMspIMspJIMwoINaeINarINb.BbvCINb.BsmINb.BsrDINb.BtsINciINcoINcoI-HF™NdeINgoMIVNheINheI-HF™NlaIIINlaIVNmeAIIINotINotI-HF™NruINsiINspINt.AlwINt.BbvCINt.BsmAINt.BspQINt.BstNBINt.CviPIIPacIPaeR7IPciIPflFIPflMIPhoIPleIPmeIPmlIPpuMIPshAIPsiIPspGIPspOMIPspXIPstIPstI-HF™PvuIPvuI-HF™PvuIIPvuII-HF™RsaIRsrIISacISacI-HF™SacIISalISalI-HF™SapISau3AISau96ISbfISbfI-HF™ScaIScaI-HF™ScrFISexAISfaNISfcISfiISfoISgrAISmaISmlISnaBISpeISpeI-HF™SphISphI-HF™SspISspI-HF™StuIStyD4IStyIStyI-HF™SwaITaqαITfiITliITseITsp45ITsp509ITspMITspRITth111IXbaIXcmIXhoIXmaIXmnIZraI    Select 2nd enzyme:      AatIIAcc65IAccIAciIAclIAcuIAfeIAflIIAflIIIAgeIAgeI-HF™AhdIAleIAluIAlwIAlwNIApaIApaLIApeKIApoIAscIAseIAsiSIAvaIAvaIIAvrIIBaeGIBaeIBamHIBamHI-HF™BanIBanIIBbsIBbvCIBbvIBccIBceAIBcgIBciVIBclIBcoDIBfaIBfuAIBfuCIBglIBglIIBlpIBmgBIBmrIBmtIBmtI-HF™BpmIBpu10IBpuEIBsaAIBsaBIBsaHIBsaIBsaI-HF™BsaJIBsaWIBsaXIBseRIBseYIBsgIBsiEIBsiHKAIBsiWIBslIBsmAIBsmBIBsmFIBsmIBsoBIBsp1286IBspCNIBspDIBspEIBspHIBspMIBspQIBsrBIBsrDIBsrFIBsrGIBsrIBssHIIBssKIBssSIBstAPIBstBIBstEIIBstEII-HF™BstNIBstUIBstXIBstYIBstZ17IBsu36IBtgIBtgZIBtsCIBtsIBtsIMutICac8IClaICspCICviAIICviKI-1CviQIDdeIDpnIDpnIIDraIDraIIIDraIII-HF™DrdIEaeIEagIEagI-HF™EarIEciIEco53kIEcoNIEcoO109IEcoP15IEcoRIEcoRI-HF™EcoRVEcoRV-HF™FatIFauIFnu4HIFokIFseIFspEIFspIHaeIIHaeIIIHgaIHhaIHincIIHindIIIHindIII-HF™HinfIHinP1IHpaIHpaIIHphIHpy166IIHpy188IHpy188IIIHpy99IHpyAVHpyCH4IIIHpyCH4IVHpyCH4VKasIKpnIKpnI-HF™LpnPIMboIMboIIMfeIMfeI-HF™MluCIMluIMlyIMmeIMnlIMscIMseIMslIMspA1IMspIMspJIMwoINaeINarINb.BbvCINb.BsmINb.BsrDINb.BtsINciINcoINcoI-HF™NdeINgoMIVNheINheI-HF™NlaIIINlaIVNmeAIIINotINotI-HF™NruINsiINspINt.AlwINt.BbvCINt.BsmAINt.BspQINt.BstNBINt.CviPIIPacIPaeR7IPciIPflFIPflMIPhoIPleIPmeIPmlIPpuMIPshAIPsiIPspGIPspOMIPspXIPstIPstI-HF™PvuIPvuI-HF™PvuIIPvuII-HF™RsaIRsrIISacISacI-HF™SacIISalISalI-HF™SapISau3AISau96ISbfISbfI-HF™ScaIScaI-HF™ScrFISexAISfaNISfcISfiISfoISgrAISmaISmlISnaBISpeISpeI-HF™SphISphI-HF™SspISspI-HF™StuIStyD4IStyIStyI-HF™SwaITaqαITfiITliITseITsp45ITsp509ITspMITspRITth111IXbaIXcmIXhoIXmaIXmnIZraI