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Request Technical Support Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home >  Double Digest Finder Double Digest Finder Cleaving a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Use this tool to select reaction conditions amenable to any two NEB restriction enzymes. Click here for more information on double digests and a chart of double digests for some common enzyme combinations. To perform a double digest calculation, please select both 1st and 2nd enzymes. Select 1st enzyme:    AatIIAcc65IAccIAciIAclIAcuIAfeIAflIIAflIIIAgeIAhdIAleIAluIAlwIAlwNIApaIApaLIApeKIApoIAscIAseIAsiSIAvaIAvaIIAvrIIBaeGIBaeIBamHIBanIBanIIBbsIBbvCIBbvIBccIBceAIBcgIBciVIBclIBfaIBfuAIBfuCIBglIBglIIBlpIBmgBIBmrIBmtIBpmIBpu10IBpuEIBsaAIBsaBIBsaHIBsaIBsaJIBsaWIBsaXIBseRIBseYIBsgIBsiEIBsiHKAIBsiWIBslIBsmAIBsmBIBsmFIBsmIBsoBIBsp1286IBspCNIBspDIBspEIBspHIBspMIBspQIBsrBIBsrDIBsrFIBsrGIBsrIBssHIIBssKIBssSIBstAPIBstBIBstEIIBstNIBstUIBstXIBstYIBstZ17IBsu36IBtgIBtgZIBtsCIBtsICac8IClaICspCICviAIICviKI-1CviQIDdeIDpnIDpnIIDraIDraIIIDrdIEaeIEagIEarIEciIEcoNIEcoO109IEcoP15IEcoRIEcoRVFatIFauIFnu4HIFokIFseIFspIHaeIIHaeIIIHgaIHhaIHincIIHindIIIHinfIHinP1IHpaIHpaIIHphIHpy166IIHpy188IHpy188IIIHpy99IHpyAVHpyCH4IIIHpyCH4IVHpyCH4VKasIKpnIMboIMboIIMfeIMluIMlyIMmeIMnlIMscIMseIMslIMspA1IMspIMwoINaeINarINb.BbvCINb.BsmINb.BsrDINb.BtsINciINcoINdeINgoMIVNheINheI-HF™NlaIIINlaIVNmeAIIINotINruINsiINspINt.AlwINt.BbvCINt.BspQINt.BstNBINt.CviPIIPacIPaeR7IPciIPflFIPflMIPhoIPleIPmeIPmlIPpuMIPshAIPsiIPspGIPspOMIPspXIPstIPvuIPvuIIPvuII-HF™RsaIRsrIISacISacIISalISalI-HF™SapISau3AISau96ISbfIScaIScaI-HF™ScrFISexAISfaNISfcISfiISfoISgrAISmaISmlISnaBISpeISphISphI-HF™SspIStuIStyD4IStyISwaITaqαITfiITliITseITsp45ITsp509ITspMITspRITth111IXbaIXcmIXhoIXmaIXmnIZraI      Select 2nd enzyme:    AatIIAcc65IAccIAciIAclIAcuIAfeIAflIIAflIIIAgeIAhdIAleIAluIAlwIAlwNIApaIApaLIApeKIApoIAscIAseIAsiSIAvaIAvaIIAvrIIBaeGIBaeIBamHIBanIBanIIBbsIBbvCIBbvIBccIBceAIBcgIBciVIBclIBfaIBfuAIBfuCIBglIBglIIBlpIBmgBIBmrIBmtIBpmIBpu10IBpuEIBsaAIBsaBIBsaHIBsaIBsaJIBsaWIBsaXIBseRIBseYIBsgIBsiEIBsiHKAIBsiWIBslIBsmAIBsmBIBsmFIBsmIBsoBIBsp1286IBspCNIBspDIBspEIBspHIBspMIBspQIBsrBIBsrDIBsrFIBsrGIBsrIBssHIIBssKIBssSIBstAPIBstBIBstEIIBstNIBstUIBstXIBstYIBstZ17IBsu36IBtgIBtgZIBtsCIBtsICac8IClaICspCICviAIICviKI-1CviQIDdeIDpnIDpnIIDraIDraIIIDrdIEaeIEagIEarIEciIEcoNIEcoO109IEcoP15IEcoRIEcoRVFatIFauIFnu4HIFokIFseIFspIHaeIIHaeIIIHgaIHhaIHincIIHindIIIHinfIHinP1IHpaIHpaIIHphIHpy166IIHpy188IHpy188IIIHpy99IHpyAVHpyCH4IIIHpyCH4IVHpyCH4VKasIKpnIMboIMboIIMfeIMluIMlyIMmeIMnlIMscIMseIMslIMspA1IMspIMwoINaeINarINb.BbvCINb.BsmINb.BsrDINb.BtsINciINcoINdeINgoMIVNheINheI-HF™NlaIIINlaIVNmeAIIINotINruINsiINspINt.AlwINt.BbvCINt.BspQINt.BstNBINt.CviPIIPacIPaeR7IPciIPflFIPflMIPhoIPleIPmeIPmlIPpuMIPshAIPsiIPspGIPspOMIPspXIPstIPvuIPvuIIPvuII-HF™RsaIRsrIISacISacIISalISalI-HF™SapISau3AISau96ISbfIScaIScaI-HF™ScrFISexAISfaNISfcISfiISfoISgrAISmaISmlISnaBISpeISphISphI-HF™SspIStuIStyD4IStyISwaITaqαITfiITliITseITsp45ITsp509ITspMITspRITth111IXbaIXcmIXhoIXmaIXmnIZraI