FAQ: What is the maximum length of cDNA product produced by WarmStart® RTx?

WarmStart RTx was developed for best performance with short amplicons in diagnostic amplification reactions (e.g. RT-LAMP and RT-PCR). However, it performs well in cDNA synthesis, with products up to 5 kb. For longer products, we recommend ProtoScript® II (NEB #M0368) or the convenient ProtoScript II First Strand cDNA Synthesis Kit (NEB #E6560).

For the longest cDNA products, we would recommend Induro® Reverse Transcriptase (NEB #M0681), a group II intron-encoded RT.