FAQ: What is the maximum length of cDNA product produced by RTx WarmStart®?

RTx WarmStart® was developed for best performance with short amplicons in diagnostic amplification reactions (e.g. RT-LAMP and RT-PCR). However, it performs well in cDNA synthesis, with products up to 5 kb. For longer products, we recommend ProtoScript® II (NEB# M0368) or the convenient ProtoScript II First Strand cDNA Synthesis Kit (NEB# E6560).