Are SHuffle strains temperature sensitive?

SHuffle® express cells are sensitive to elevated temperatures and do no grow at 42º C, however, at temperatures 37º C and below, both SHuffle express (E. coli B) and SHuffle (E. coli K12) cells are viable. We therefore suggest growing SHuffle cells at non-stressed temperatures of 30º C and induce expression of your protein at even lower temperatures.