Home FAQs Can I clone my protein as a fusion to the N- or C-terminus of the tags?

FAQ: Can I clone my protein as a fusion to the N- or C-terminus of the tags?

Yes. SNAP- and CLIP-tags can be fused to either the N- or C-terminus of a protein of interest. However, to label surface proteins on the outside of cells using SNAP-, CLIP- the tag must be cloned so that it is oriented to the extracellular surface of the plasma membrane. In this orientation, the tag is accessible to its fluorophore conjugated substrate.