FAQ: Is there variability in the cleavage site of HphI?

HphI has been shown to cleave at N9/N8 depending on the sequence between the recognition and cleavage sites. (Kang, C., Wu, C. (1987) NAR, 15, 2279-2294.; Kang, C., Cho, S. (1990) Mol. Cells 181-186.)

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